Multilabel plate reader
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PRESENTATION
VICTOR3 is a multilabel, multitask plate reader for all light-emitting and light-absorbing detection technologies including: fluorescence (top and bottom), luminescence, absorbance, UV absorbance, time-resolved fluorometry, and fluorescence polarization. VICTOR3 can operate as a stand-alone instrument or can integrate into a robotic system.
Room 8.1.74A
Computer name: Janus / 10.101.93.49 (why?)
Absorbance / Fluorescence excitation
* 405/20 nm
* 450/20 nm
* 490/15 nm
* 595/20 nm
* 485/25 nm
* 350/60 nm
* 480/40 nm
* 531/40 nm
(emission center / bandwidth)
* 405/20 nm
* 450/20 nm
* 490/15 nm
* 595/20 nm
* 485/25 nm
* 350/60 nm
* 480/40 nm
* 531/40 nm
(emission center / bandwidth)
Fluorescence emission
* 460 nm
* 510 nm
* 520 nm
* 535 nm
* 545 nm
* 572 nm
* 595 nm
* 615 nm
* 642 nm
* 665 nm
Luminescence
- Continuous light source (cw) for fluorometric and photometric measurements: Tungsten-halogen lamp, 75 W, spectral range 340-800 nm. Changeable rotatable filter wheels, provided with 8 or 4 positions for 15 or 25.4 mm filters.
- Flash light source for TR-fluorometric and UV absorbance measurements: UV Xenon flash tube 1500 V. Filter slide provided with three positions.
- R 1527P photomultiplier tube: for fluorometry, luminometry and TR-fluorometry
- Photo diode: for photometry
- Turn on the computer and Victor (the power button is located on the back side).
- Open the “Wallac 1420” software using the desktop icon
- Select a protocol from the list
- Place the multi-well plate on the plate reader (right side compartment).
- Click “start”
- Acquisition will start
- Clicking the “Live display” tab will display a heatmap of the data as it is acquired. The numeric range and scale type (linear/logarithmic) can be adjusted.
- Results from the current and previous assays can be accessed through the menu: “Tools > Explorer”. Assays are named with a sequential number. The acquisition date/time is also shown.
- Data is shown in tabular and matrix (2D) format. Use “File > Save as” to save as plain text or Excel format.
BioISI users: 0€ /h
External Users with BioISI collaboration: 3€/h
External Users: 5€/h
EQUIPMENT RESPONSIBLE
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| Hugo Botelho
BioISI hmbotelho@fc.ul.pt Room 8.1.76 |