Publications with work performed at FCULMF, 2015 to present:

  1. Quaresma, M. C., Pankonien, I., Clarke, L. A., Sousa, L. S., Silva, I. A. L., Railean, V., … Amaral, M. D. (2020). Mutant CFTR Drives TWIST1 mediated epithelial-mesenchymal transition. Cell Death & Disease, 11(10), 920.
  2. Santos, J. D., Pinto, F. R., Ferreira, J. F., Amaral, M. D., Zaccolo, M., & Farinha, C. M. (2020). Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation. The Biochemical Journal, 477(13), 2561–2580.
  3. Lopes-Pacheco, M., Silva, I. A. L., Turner, M. J., Carlile, G. W., Sondo, E., Thomas, D. Y., … Amaral, M. D. (2020). Characterization of the mechanism of action of RDR01752, a novel corrector of F508del-CFTR. Biochemical Pharmacology, 180, 114133.
  4. Marques, N. T., Filipe, A., Pinto, P., Barroso, J., Trindade, H., Power, D. M., & Figueiredo, A. C. (2020). Trichome Density in Relation to Volatiles Emission and 1,8-Cineole Synthase Gene Expression in Thymus albicans Vegetative and Reproductive Organs. Chemistry & Biodiversity, 17(3), e1900669.
  5. Cortinhas, A., Caperta, A. D., Teixeira, G., Carvalho, L., & Abreu, M. M. (2020). Harnessing sediments of coastal aquaculture ponds through technosols construction for halophyte cultivation using saline water irrigation. Journal of Environmental Management, 261, 109907.
  6. Khmelinskaia, A., Marquês, J. M. T., Bastos, A. E. P., Antunes, C. A. C., Bento-Oliveira, A., Scolari, S., … de Almeida, R. F. M. (2020). Liquid-Ordered Phase Formation by Mammalian and Yeast Sterols: A Common Feature With Organizational Differences. Frontiers in Cell and Developmental Biology, 8, 337.
  7. Caperta, A. D., Róis, A. S., Teixeira, G., Garcia‐Caparros, P., & Flowers, T. J. (2020). Secretory structures in plants: Lessons from the Plumbaginaceae on their origin, evolution and roles in stress tolerance. Plant, Cell & Environment, 43(12), 2912–2931.
  8. Correia, S., Santos, M., Glińska, S., Gapińska, M., Matos, M., Carnide, V., … Gonçalves, B. (2020). Effects of exogenous compound sprays on cherry cracking: skin properties and gene expression. Journal of the Science of Food and Agriculture, 100(7), 2911–2921.
  9. Sousa, L., Pankonien, I., Clarke, L. A., Silva, I., Kunzelmann, K., & Amaral, M. D. (2020). KLF4 Acts as a wt-CFTR Suppressor through an AKT-Mediated Pathway. Cells, 9(7).
  10. Vonk, A. M., van Mourik, P., Ramalho, A. S., Silva, I. A. L., Statia, M., Kruisselbrink, E., … Beekman, J. M. (2020). Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids. STAR Protocols, 1(1), 100019.
  11. Sereno, M., Haskó, J., Molnár, K., Medina, S. J., Reisz, Z., Malhó, R., … Brito, M. A. (2020). Downregulation of circulating miR 802-5p and miR 194-5p and upregulation of brain MEF2C along breast cancer brain metastasization. Molecular Oncology, 14(3), 520–538.
  12. Silva, I. A. L., Doušová, T., Ramalho, S., Centeio, R., Clarke, L. A., Railean, V., … Amaral, M. D. (2020). Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G. Biochimica et Biophysica Acta. Molecular Basis of Disease, 1866(11), 165905.
  13. Pinto, M. C., Schreiber, R., Lerias, J., Ousingsawat, J., Duarte, A., Amaral, M., & Kunzelmann, K. (2020). Regulation of TMEM16A by CK2 and Its Role in Cellular Proliferation. Cells, 9(5).
  14. Silva, I. A. L., Duarte, A., Marson, F. A. L., Centeio, R., Doušová, T., Kunzelmann, K., & Amaral, M. D. (2020). Assessment of Distinct Electrophysiological Parameters in Rectal Biopsies for the Choice of the Best Diagnosis/Prognosis Biomarkers for Cystic Fibrosis. Frontiers in Physiology, 11.
  15. Amaral, M. D., & Beekman, J. M. (2020). Activating alternative chloride channels to treat CF: Friends or Foes?: Report on the Meeting of the Basic Science Working Group in Dubrovnik, Croatia. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 19(1), 11–15.
  16. Amaral, M. D., Hutt, D. M., Tomati, V., Botelho, H. M., & Pedemonte, N. (2020). CFTR processing, trafficking and interactions. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 19 Suppl 1, S33–S36.
  17. Simão, M., Moço, M., Marques, L., & Santos, R. (2020). Characterization of the glycans involved in sea urchin Paracentrotus lividus reversible adhesion. Marine Biology, 167(9), 125.
  18. Vaz Dias, F., Serrazina, S., Vitorino, M., Marchese, D., Heilmann, I., Godinho, M., … Malhó, R. (2019). A role for diacylglycerol kinase 4 in signalling crosstalk during Arabidopsis pollen tube growth. New Phytologist, 222(3), 1434–1446.
  19. Palma, E., Botelho, H. M., Morais, G. R., Rodrigues, I., Santos, I. C., Campello, M. P. C., … Paulo, A. (2019). Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies. JBIC Journal of Biological Inorganic Chemistry, 24(1), 71–89.
  20. Awatade, N. T., Ramalho, S., Silva, I. A. L., Felício, V., Botelho, H. M., de Poel, E., … Amaral, M. D. (2019). R560S: A class II CFTR mutation that is not rescued by current modulators. Journal of Cystic Fibrosis, 18(2), 182–189.
  21. Santos, J. D., Canato, S., Carvalho, A. S., Botelho, H. M., Aloria, K., Amaral, M. D., … Farinha, C. M. (2019). Folding Status Is Determinant over Traffic-Competence in Defining CFTR Interactors in the Endoplasmic Reticulum. Cells, 8(4), 353.
  22. Loureiro, C. A., Santos, J. D., Matos, A. M., Jordan, P., Matos, P., Farinha, C. M., & Pinto, F. R. (2019). Network Biology Identifies Novel Regulators of CFTR Trafficking and Membrane Stability. Frontiers in Pharmacology, 10.
  23. Patel, W., Moore, P. J., Sassano, M. F., Lopes-Pacheco, M., Aleksandrov, A. A., Amaral, M. D., … Gray, M. A. (2019). Increases in cytosolic Ca2+ induce dynamin- and calcineurin-dependent internalisation of CFTR. Cellular and Molecular Life Sciences, 76(5), 977–994.
  24. Simões, F. B., Quaresma, M. C., Clarke, L. A., Silva, I. Al, Pankonien, I., Railean, V., … Amaral, M. D. (2019). TMEM16A chloride channel does not drive mucus production. Life Science Alliance, 2(6).
  25. Olivença, D. V., Uliyakina, I., Fonseca, L. L., Amaral, M. D., Voit, E. O., & Pinto, F. R. (2018). A Mathematical Model of the Phosphoinositide Pathway. Scientific Reports, 8(1), 3904.
  26. Sampaio-Pinto, V., Rodrigues, S. C., Laundos, T. L., Silva, E. D., Vasques-Nóvoa, F., Silva, A. C., … Nascimento, D. S. (2018). Neonatal Apex Resection Triggers Cardiomyocyte Proliferation, Neovascularization and Functional Recovery Despite Local Fibrosis. Stem Cell Reports, 10(3), 860–874.
  27. Casimiro, M. H., Gomes, S. R., Rodrigues, G., Leal, J. P., & Ferreira, L. M. (2018). Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies. Materials (Basel, Switzerland), 11(12).
  28. Lérias, J. R., Pinto, M. C., Botelho, H. M., Awatade, N. T., Quaresma, M. C., Silva, I. A. L., … Amaral, M. D. (2018). A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research, 1865(2), 421–431.
  29. Sebastiana, M., da Silva, A. B., Matos, A. R., Alcântara, A., Silvestre, S., & Malhó, R. (2018). Ectomycorrhizal inoculation with Pisolithus tinctorius reduces stress induced by drought in cork oak. Mycorrhiza, 28(3), 247–258.
  30. Camelo, C., Vilas-Boas, F., Cepeda, A. P., Real, C., Barros-Martins, J., Pinto, F., … Cyrne, L. (2017). Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae. Yeast, 34(9), 383–395.
  31. Gomes, S., Rodrigues, G., Martins, G., Henriques, C., & Silva, J. C. (2017). Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering. International Journal of Biological Macromolecules, 102, 1174–1185.
  32. Nunes, A. M., Wuebbles, R. D., Sarathy, A., Fontelonga, T. M., Deries, M., Burkin, D. J., & Thorsteinsdóttir, S. (2017). Impaired fetal muscle development and JAK-STAT activation mark disease onset and progression in a mouse model for merosin-deficient congenital muscular dystrophy. Human Molecular Genetics, 26(11), 2018–2033.
  33. Igreja, S., Clarke, L. A., Botelho, H. M., Marques, L., & Amaral, M. D. (2016). Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides. Human Mutation, 37(2), 209–215.
  34. Gonçalves, A. B., Thorsteinsdóttir, S., & Deries, M. (2016). Rapid and simple method for in vivo ex utero development of mouse embryo explants. Differentiation, 91(4–5), 57–67.
  35. Amaral, M. D., Farinha, C. M., Matos, P., & Botelho, H. M. (2016). Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Methods in Molecular Biology (Clifton, N.J.), 1459, 105–126.
  36. Lebesgue, N., da Costa, G., Ribeiro, R. M., Ribeiro-Silva, C., Martins, G. G., Matranga, V., … Santos, R. (2016). Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach. Journal of Proteomics, 138, 61–71.
  37. Silva, A. C., Rodrigues, S. C., Caldeira, J., Nunes, A. M., Sampaio-Pinto, V., Resende, T. P., … Pinto-do-Ó, P. (2016). Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult. Biomaterials, 104, 52–64.
  38. Luzio, A., Matos, M., Santos, D., Fontaínhas-Fernandes, A. A., Monteiro, S. M., & Coimbra, A. M. (2016). Disruption of apoptosis pathways involved in zebrafish gonad differentiation by 17α-ethinylestradiol and fadrozole exposures. Aquatic Toxicology, 177, 269–284.
  39. Ramalho, A. S., Clarke, L. A., Sousa, M., Felicio, V., Barreto, C., Lopes, C., & Amaral, M. D. (2016). Comparative ex vivo, in vitro and in silico analyses of a CFTR splicing mutation: Importance of functional studies to establish disease liability of mutations. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 15(1), 21–33.
  40. Saavedra, L., Catarino, R., Heinz, T., Heilmann, I., Bezanilla, M., & Malho, R. M. (2015). Phosphatase and tensin homolog (PTEN) is a growth repressor of both rhizoid and gametophore development in the moss Physcomitrella patens. Plant Physiology, pp.01197.2015.
  41. Botelho, H. M., Uliyakina, I., Awatade, N. T., Proença, M. C., Tischer, C., Sirianant, L., … Amaral, M. D. (2015). Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery. Scientific Reports, 5(1), 9038.
  42. Clarke, L. A., Botelho, H. M., Sousa, L., Falcao, A. O., & Amaral, M. D. (2015). Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators. Genomics, 106(5), 268–277.
  43. Loureiro, C. A., Matos, A. M., Dias-Alves, Â., Pereira, J. F., Uliyakina, I., Barros, P., … Matos, P. (2015). A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint. Science Signaling, 8(377), ra48.
  44. Verkman, A. S., Edelman, A., Amaral, M., Mall, M. A., Beekman, J. M., Meiners, T., … Bear, C. E. (2015). Finding new drugs to enhance anion secretion in cystic fibrosis: Toward suitable systems for better drug screening. Report on the pre-conference meeting to the 12th ECFS Basic Science Conference, Albufeira, 25-28 March 2015. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 14(6), 700–705.
  45. Amaral, M. D., & Balch, W. E. (2015). Hallmarks of therapeutic management of the cystic fibrosis functional landscape. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 14(6), 687–699.