Publications

Publications with work performed at FCULMF, 2015 to present:

  1. Quaresma, M. C., Pankonien, I., Clarke, L. A., Sousa, L. S., Silva, I. A. L., Railean, V., … Amaral, M. D. (2020). Mutant CFTR Drives TWIST1 mediated epithelial-mesenchymal transition. Cell Death & Disease, 11(10), 920. https://doi.org/10.1038/s41419-020-03119-z
  2. Santos, J. D., Pinto, F. R., Ferreira, J. F., Amaral, M. D., Zaccolo, M., & Farinha, C. M. (2020). Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation. The Biochemical Journal, 477(13), 2561–2580. https://doi.org/10.1042/BCJ20200287
  3. Lopes-Pacheco, M., Silva, I. A. L., Turner, M. J., Carlile, G. W., Sondo, E., Thomas, D. Y., … Amaral, M. D. (2020). Characterization of the mechanism of action of RDR01752, a novel corrector of F508del-CFTR. Biochemical Pharmacology, 180, 114133. https://doi.org/10.1016/j.bcp.2020.114133
  4. Marques, N. T., Filipe, A., Pinto, P., Barroso, J., Trindade, H., Power, D. M., & Figueiredo, A. C. (2020). Trichome Density in Relation to Volatiles Emission and 1,8-Cineole Synthase Gene Expression in Thymus albicans Vegetative and Reproductive Organs. Chemistry & Biodiversity, 17(3), e1900669. https://doi.org/10.1002/cbdv.201900669
  5. Cortinhas, A., Caperta, A. D., Teixeira, G., Carvalho, L., & Abreu, M. M. (2020). Harnessing sediments of coastal aquaculture ponds through technosols construction for halophyte cultivation using saline water irrigation. Journal of Environmental Management, 261, 109907. https://doi.org/10.1016/j.jenvman.2019.109907
  6. Khmelinskaia, A., Marquês, J. M. T., Bastos, A. E. P., Antunes, C. A. C., Bento-Oliveira, A., Scolari, S., … de Almeida, R. F. M. (2020). Liquid-Ordered Phase Formation by Mammalian and Yeast Sterols: A Common Feature With Organizational Differences. Frontiers in Cell and Developmental Biology, 8, 337. https://doi.org/10.3389/fcell.2020.00337
  7. Caperta, A. D., Róis, A. S., Teixeira, G., Garcia‐Caparros, P., & Flowers, T. J. (2020). Secretory structures in plants: Lessons from the Plumbaginaceae on their origin, evolution and roles in stress tolerance. Plant, Cell & Environment, 43(12), 2912–2931. https://doi.org/10.1111/pce.13825
  8. Correia, S., Santos, M., Glińska, S., Gapińska, M., Matos, M., Carnide, V., … Gonçalves, B. (2020). Effects of exogenous compound sprays on cherry cracking: skin properties and gene expression. Journal of the Science of Food and Agriculture, 100(7), 2911–2921. https://doi.org/10.1002/jsfa.10318
  9. Sousa, L., Pankonien, I., Clarke, L. A., Silva, I., Kunzelmann, K., & Amaral, M. D. (2020). KLF4 Acts as a wt-CFTR Suppressor through an AKT-Mediated Pathway. Cells, 9(7). https://doi.org/10.3390/cells9071607
  10. Vonk, A. M., van Mourik, P., Ramalho, A. S., Silva, I. A. L., Statia, M., Kruisselbrink, E., … Beekman, J. M. (2020). Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids. STAR Protocols, 1(1), 100019. https://doi.org/10.1016/j.xpro.2020.100019
  11. Sereno, M., Haskó, J., Molnár, K., Medina, S. J., Reisz, Z., Malhó, R., … Brito, M. A. (2020). Downregulation of circulating miR 802-5p and miR 194-5p and upregulation of brain MEF2C along breast cancer brain metastasization. Molecular Oncology, 14(3), 520–538. https://doi.org/10.1002/1878-0261.12632
  12. Silva, I. A. L., Doušová, T., Ramalho, S., Centeio, R., Clarke, L. A., Railean, V., … Amaral, M. D. (2020). Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G. Biochimica et Biophysica Acta. Molecular Basis of Disease, 1866(11), 165905. https://doi.org/10.1016/j.bbadis.2020.165905
  13. Pinto, M. C., Schreiber, R., Lerias, J., Ousingsawat, J., Duarte, A., Amaral, M., & Kunzelmann, K. (2020). Regulation of TMEM16A by CK2 and Its Role in Cellular Proliferation. Cells, 9(5). https://doi.org/10.3390/cells9051138
  14. Silva, I. A. L., Duarte, A., Marson, F. A. L., Centeio, R., Doušová, T., Kunzelmann, K., & Amaral, M. D. (2020). Assessment of Distinct Electrophysiological Parameters in Rectal Biopsies for the Choice of the Best Diagnosis/Prognosis Biomarkers for Cystic Fibrosis. Frontiers in Physiology, 11. https://doi.org/10.3389/fphys.2020.604580
  15. Amaral, M. D., & Beekman, J. M. (2020). Activating alternative chloride channels to treat CF: Friends or Foes?: Report on the Meeting of the Basic Science Working Group in Dubrovnik, Croatia. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 19(1), 11–15. https://doi.org/10.1016/j.jcf.2019.10.005
  16. Amaral, M. D., Hutt, D. M., Tomati, V., Botelho, H. M., & Pedemonte, N. (2020). CFTR processing, trafficking and interactions. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 19 Suppl 1, S33–S36. https://doi.org/10.1016/j.jcf.2019.10.017
  17. Simão, M., Moço, M., Marques, L., & Santos, R. (2020). Characterization of the glycans involved in sea urchin Paracentrotus lividus reversible adhesion. Marine Biology, 167(9), 125. https://doi.org/10.1007/s00227-020-03707-9
  18. Vaz Dias, F., Serrazina, S., Vitorino, M., Marchese, D., Heilmann, I., Godinho, M., … Malhó, R. (2019). A role for diacylglycerol kinase 4 in signalling crosstalk during Arabidopsis pollen tube growth. New Phytologist, 222(3), 1434–1446. https://doi.org/10.1111/nph.15674
  19. Palma, E., Botelho, H. M., Morais, G. R., Rodrigues, I., Santos, I. C., Campello, M. P. C., … Paulo, A. (2019). Unravelling the antitumoral potential of novel bis(thiosemicarbazonato) Zn(II) complexes: structural and cellular studies. JBIC Journal of Biological Inorganic Chemistry, 24(1), 71–89. https://doi.org/10.1007/s00775-018-1629-6
  20. Awatade, N. T., Ramalho, S., Silva, I. A. L., Felício, V., Botelho, H. M., de Poel, E., … Amaral, M. D. (2019). R560S: A class II CFTR mutation that is not rescued by current modulators. Journal of Cystic Fibrosis, 18(2), 182–189. https://doi.org/10.1016/j.jcf.2018.07.001
  21. Santos, J. D., Canato, S., Carvalho, A. S., Botelho, H. M., Aloria, K., Amaral, M. D., … Farinha, C. M. (2019). Folding Status Is Determinant over Traffic-Competence in Defining CFTR Interactors in the Endoplasmic Reticulum. Cells, 8(4), 353. https://doi.org/10.3390/cells8040353
  22. Loureiro, C. A., Santos, J. D., Matos, A. M., Jordan, P., Matos, P., Farinha, C. M., & Pinto, F. R. (2019). Network Biology Identifies Novel Regulators of CFTR Trafficking and Membrane Stability. Frontiers in Pharmacology, 10. https://doi.org/10.3389/fphar.2019.00619
  23. Patel, W., Moore, P. J., Sassano, M. F., Lopes-Pacheco, M., Aleksandrov, A. A., Amaral, M. D., … Gray, M. A. (2019). Increases in cytosolic Ca2+ induce dynamin- and calcineurin-dependent internalisation of CFTR. Cellular and Molecular Life Sciences, 76(5), 977–994. https://doi.org/10.1007/s00018-018-2989-3
  24. Simões, F. B., Quaresma, M. C., Clarke, L. A., Silva, I. Al, Pankonien, I., Railean, V., … Amaral, M. D. (2019). TMEM16A chloride channel does not drive mucus production. Life Science Alliance, 2(6). https://doi.org/10.26508/lsa.201900462
  25. Olivença, D. V., Uliyakina, I., Fonseca, L. L., Amaral, M. D., Voit, E. O., & Pinto, F. R. (2018). A Mathematical Model of the Phosphoinositide Pathway. Scientific Reports, 8(1), 3904. https://doi.org/10.1038/s41598-018-22226-8
  26. Sampaio-Pinto, V., Rodrigues, S. C., Laundos, T. L., Silva, E. D., Vasques-Nóvoa, F., Silva, A. C., … Nascimento, D. S. (2018). Neonatal Apex Resection Triggers Cardiomyocyte Proliferation, Neovascularization and Functional Recovery Despite Local Fibrosis. Stem Cell Reports, 10(3), 860–874. https://doi.org/10.1016/j.stemcr.2018.01.042
  27. Casimiro, M. H., Gomes, S. R., Rodrigues, G., Leal, J. P., & Ferreira, L. M. (2018). Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies. Materials (Basel, Switzerland), 11(12). https://doi.org/10.3390/ma11122535
  28. Lérias, J. R., Pinto, M. C., Botelho, H. M., Awatade, N. T., Quaresma, M. C., Silva, I. A. L., … Amaral, M. D. (2018). A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research, 1865(2), 421–431. https://doi.org/10.1016/j.bbamcr.2017.11.009
  29. Sebastiana, M., da Silva, A. B., Matos, A. R., Alcântara, A., Silvestre, S., & Malhó, R. (2018). Ectomycorrhizal inoculation with Pisolithus tinctorius reduces stress induced by drought in cork oak. Mycorrhiza, 28(3), 247–258. https://doi.org/10.1007/s00572-018-0823-2
  30. Camelo, C., Vilas-Boas, F., Cepeda, A. P., Real, C., Barros-Martins, J., Pinto, F., … Cyrne, L. (2017). Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae. Yeast, 34(9), 383–395. https://doi.org/10.1002/yea.3240
  31. Gomes, S., Rodrigues, G., Martins, G., Henriques, C., & Silva, J. C. (2017). Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering. International Journal of Biological Macromolecules, 102, 1174–1185. https://doi.org/10.1016/j.ijbiomac.2017.05.004
  32. Nunes, A. M., Wuebbles, R. D., Sarathy, A., Fontelonga, T. M., Deries, M., Burkin, D. J., & Thorsteinsdóttir, S. (2017). Impaired fetal muscle development and JAK-STAT activation mark disease onset and progression in a mouse model for merosin-deficient congenital muscular dystrophy. Human Molecular Genetics, 26(11), 2018–2033. https://doi.org/10.1093/hmg/ddx083
  33. Igreja, S., Clarke, L. A., Botelho, H. M., Marques, L., & Amaral, M. D. (2016). Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides. Human Mutation, 37(2), 209–215. https://doi.org/10.1002/humu.22931
  34. Gonçalves, A. B., Thorsteinsdóttir, S., & Deries, M. (2016). Rapid and simple method for in vivo ex utero development of mouse embryo explants. Differentiation, 91(4–5), 57–67. https://doi.org/10.1016/j.diff.2015.12.002
  35. Amaral, M. D., Farinha, C. M., Matos, P., & Botelho, H. M. (2016). Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Methods in Molecular Biology (Clifton, N.J.), 1459, 105–126. https://doi.org/10.1007/978-1-4939-3804-9_7
  36. Lebesgue, N., da Costa, G., Ribeiro, R. M., Ribeiro-Silva, C., Martins, G. G., Matranga, V., … Santos, R. (2016). Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach. Journal of Proteomics, 138, 61–71. https://doi.org/10.1016/j.jprot.2016.02.026
  37. Silva, A. C., Rodrigues, S. C., Caldeira, J., Nunes, A. M., Sampaio-Pinto, V., Resende, T. P., … Pinto-do-Ó, P. (2016). Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult. Biomaterials, 104, 52–64. https://doi.org/10.1016/j.biomaterials.2016.06.062
  38. Luzio, A., Matos, M., Santos, D., Fontaínhas-Fernandes, A. A., Monteiro, S. M., & Coimbra, A. M. (2016). Disruption of apoptosis pathways involved in zebrafish gonad differentiation by 17α-ethinylestradiol and fadrozole exposures. Aquatic Toxicology, 177, 269–284. https://doi.org/10.1016/j.aquatox.2016.05.029
  39. Ramalho, A. S., Clarke, L. A., Sousa, M., Felicio, V., Barreto, C., Lopes, C., & Amaral, M. D. (2016). Comparative ex vivo, in vitro and in silico analyses of a CFTR splicing mutation: Importance of functional studies to establish disease liability of mutations. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 15(1), 21–33. https://doi.org/10.1016/j.jcf.2015.02.002
  40. Saavedra, L., Catarino, R., Heinz, T., Heilmann, I., Bezanilla, M., & Malho, R. M. (2015). Phosphatase and tensin homolog (PTEN) is a growth repressor of both rhizoid and gametophore development in the moss Physcomitrella patens. Plant Physiology, pp.01197.2015. https://doi.org/10.1104/pp.15.01197
  41. Botelho, H. M., Uliyakina, I., Awatade, N. T., Proença, M. C., Tischer, C., Sirianant, L., … Amaral, M. D. (2015). Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery. Scientific Reports, 5(1), 9038. https://doi.org/10.1038/srep09038
  42. Clarke, L. A., Botelho, H. M., Sousa, L., Falcao, A. O., & Amaral, M. D. (2015). Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators. Genomics, 106(5), 268–277. https://doi.org/10.1016/j.ygeno.2015.07.005
  43. Loureiro, C. A., Matos, A. M., Dias-Alves, Â., Pereira, J. F., Uliyakina, I., Barros, P., … Matos, P. (2015). A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint. Science Signaling, 8(377), ra48. https://doi.org/10.1126/scisignal.aaa1580
  44. Verkman, A. S., Edelman, A., Amaral, M., Mall, M. A., Beekman, J. M., Meiners, T., … Bear, C. E. (2015). Finding new drugs to enhance anion secretion in cystic fibrosis: Toward suitable systems for better drug screening. Report on the pre-conference meeting to the 12th ECFS Basic Science Conference, Albufeira, 25-28 March 2015. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 14(6), 700–705. https://doi.org/10.1016/j.jcf.2015.10.001
  45. Amaral, M. D., & Balch, W. E. (2015). Hallmarks of therapeutic management of the cystic fibrosis functional landscape. Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society, 14(6), 687–699. https://doi.org/10.1016/j.jcf.2015.09.006