Inverted widefield fluorescence microscope
PRESENTATION
The Zeiss Axiovert 200 is a manual inverted widefield fluorescence microscope. Its sensitive digital camera allows the acquisition of fluorescent signals in light samples and slides. It is suitable for routine examination of cell cultures, manual image acquisition and differential interference contrast (DIC) imaging.
Room: 8.1.79 Computer name: axiovert200 / 10.101.93.112 (why?)
| Pos | Mag | NA | IM | Corr. | WD (mm) | Glass (mm) | Color Code | Obs |
|---|---|---|---|---|---|---|---|---|
| 1 | 10x | 0.25 | Air | A-PLAN | 4.5 | 0.17 | Ph1, PlasDIC | |
| 2 | 20x | 0.80 | Air | PLAN-APO | 0.55 | 0.17 | - | |
| 3 | 40x | 0.6 | Air | LD PLAN-Neofluar | 2.9 | 0-1.5 | Ph2, PlasDIC |
Pos Position
Mag Magnification
NA Numerical Aperture
IM Immersion Medium
Corr. Correction Type
WD Working Distance
Glass Coverglass
Obs Observations
| Position | Excitation (nm) | Emission (nm) | Fluorophores | Model |
|---|---|---|---|---|
| 1 | BP G365 (300-400) | BP 420-470 | DAPI, hoechst, Alexa350 | 49 |
| 2 | BP 450-490 | BP 515-565 | GFP, Alexa488, cy2, FITC | 10 |
| 3 | BP 550-580 | BP 590-650 | mCherry, RFP, cy3.5, TRITC | 31 |
| 4 | BP 625-655 | BP 665-715 | Alexa647, Alexa660, cy5, TO-PRO3 | 50 |
| 5 | - | - | empty | - |
Transmitted light
Halogen lamp
Fluorescence
High pressure mercury vapor arc-discharge lamps. Lifetime: 200 hours Includes a dedicated power supply
Monochrome CCD camera.
1.4 MP (1388 x 1040 pixels)
Pixel size 6.45 μm × 6.45 μm
Bit depth 8 bit/12 bit
One stage Peltier cooling
Spectral sensitivity range: 350-1000 nm
Integration time: 1 ms – 60 s
Universal supports for for multiwell plates and microscopy slides
Immersion oil (RI 1.5180)
COMMON USAGE PROCEDURES
BioISI users: 0 €/h
External users with BioISI collaboration: 1.5 €/h
External users: 2 €/h
EQUIPMENT RESPONSIBLE
 |
 |
| Hugo Botelho
hmbotelho@fc.ul.pt Room 8.1.76 |
Luís Marques
FCUL Microscopy technician lfmarques@fc.ul.pt Room 8.1.79 / 2.1.15 |