Olympus BX60

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PRESENTATION

BX60

Olympus BX60 manual fluorescence microscope with mercury fluorescence illuminator, and Nomarski/DIC Prism for Transmited Light


Room: 2.1.15 Computer name:


TBA


PosMagNAIMCorr.WD (mm)Glass (mm)Color CodeObs
10x0.40AirUPlanApo---
20x0.70AirUPlanApo---
40x1.00OilUPlanApo-Open iris for fluorescence
100x1.35OilUPlanApo--Open iris for fluorescence

Pos Position

Mag Magnification

NA Numerical Aperture

IM Immersion Medium

Corr. Correction Type

WD Working Distance

Glass Coverglass

Obs Observations

40× use with/out coverglass; 100× use with coverglass


PositionExcitation (nm)Emission (nm)FluorophoresModel
U-MWU2BP 330-385LP >420DAPI, hoechst-
U-MNIBBP 470-490LP >515GFP, Alexa488, cy2, FITC-
U-MNGBP 530-550LP >590RFP, YFP, Alexa546, Alexa568, cy3, TRITC-
U-MSWBBP 420-480LP >515only w/ cromossina-


Transmitted light

Halogen lamp

Fluorescence

Olympus U-RFL-T Mercury lamp


Hamamatsu Orca R2 cooled monochromatic CCD camera (1.3 Mpixel), coupled to microscope via a 1x adaptor


 ACCESSORIES


Procedure to turn system on

  1. Turn PC and monitor on only if acquiring images
  2. Turn on power switch for the transmitted light illuminator (back of microscope)
  3. Turn on fluorescence illuminator ONLY IF NECESSARY (eg. to see immunolabelled specimens)
  4. Start software at the desktop
  5. Start Hamamatsu R2 camera power box (large box sitting between the microscope and fluorescence PS)
  6. Start the HCIimage software (software will only see the camera if power box was turned on before. If not, then to the right of the “select gray or multicolor” you will see “disk”. Close HCI, start camera first.
  7. Load sample, switch the microscope to whatever fluorescence channel you want to start with, and then proceed according to the instructions below.

Notice that the microscope is not automatic, so the software WILL ask to switch filters manually during “live” preview or acquisition. Don’t forget to save images as TIFF. These can be opened with ImageJ or any other scientific image analysis software, but since they are 16bit/channel images, they may not open with other programs correctly. There is an option of saving in 8bit/JPG but I strongly advise against doing it, otherwise why use this camera?

Images are saved in 16bit format, so they should be opened with software capable of operating with 16 bit images (eg ImageJ)

Micrometer images for the R2 camera can be dowloaded from here

Procedure to turn off system:

Turn off microscope and fluorescence illuminator

Turn off camera power supply

Save your work and shutdown the PC


HCImage


 LINKS / USER MANUALS


PEOPLE RESPONSIBLE FOR THE EQUIPMENT

Telmo gabriela_rodrigues

Telmo Nunes

FCUL

Microscopy technician

mevarrimento@fc.ul.pt

Room 2.1.15

Ext: 22181

Gabriela Rodrigues

FCUL / CE3C

Assistant professor

mgrodrigues@fc.ul.pt

Room 2.3.10

Ext: 22310