Hands-on Workshop on High-Throughput Microscopy 2017

Contents Faculty and Schedule Venue
 

Lisboa (Portugal) 17 – 21 July 2017

 

Learn about the latest developments on High-Throughput Microscopy, and discuss your project with the experts


Contents

Workshop Overview
Participants will learn the basic principles of High-Throughput Microscopy (HTM) applied to RNAi and drug screening. The workshop takes the form of a small HTM project: participants will be introduced to the theory of HTM, involved in disussions on how to design a RNAi/drug screen, perform an actual HTM experiment (including a bioassay and automated image acquisition), perform image analysis and statistical data analysis. Some applications of HTM will be highlighted on research seminars.

Why apply?
Systems biology based approaches are powerful tools to understand complex cellular processes. These approaches rely on the acquisition of extensive datasets in order to characterize a given biological system. Automated high-throughput microscopy technologies allow direct visualization of phenotypes that arise from the treatment of cells/tissues with a large number of siRNAs/compounds/experimental conditions.
This workshop will provide participants with hands-on knowledge on the design, implementation, optimization, image acquisition and analysis of high-throughput microscopy-based screens.

Who should apply?
The workshop is directed at PhD Students, Post-Docs, Microscopy Technicians and Independent Researchers.
Participants are expected to be acquainted with the basics of widefield fluorescence microscopy. No prior experience with high throughput techniques is required.

The course is promoted by BioISI. The instructors are members of FCUL (BioISI, CEAUL), IGC (Advanced Imaging Lab) and the Advanced Light Microscopy Facility at EMBL.

 

Topics Tutorials Lab work
Fluorescence microscopy: limits and potentialities
Detecting light in optical systems
High – Throughput Microscopy (HTM) overview
HTM RNAi screening
Automated image acquisition
Image and Statistical analysis
HTM Assay development
siRNA design
Visual inspection and statistical analysis of HTM data
Image Analysis: ImageJ, Cell Profiler

Confocal versus wide-field systems
Live cell imaging: image acquisition and output

 

 

Faculty and Schedule

Margarida Amaral
FCUL, Lisboa
Marília Antunes
FCUL, Lisboa
Gabriel Martins
IGC, Oeiras
Rui
Malhó

FCUL, Lisboa
Luís Marques
FCUL, Lisboa
Beate Neumann
EMBL, Heidelberg
Hugo Botelho
FCUL, Lisboa
Margarida Quaresma
FCUL, Lisboa
Volker Hilsenstein
EMBL, Heidelberg

 

Workshop Schedule
For a detailed schedule in PDF format, click here

 

 

Mon, 17

Tue, 18

Wed, 19

Thu, 20

Fri21

9.0010.00

Registration & Reception
M Amaral R Malhó 

(L1)
HT Confocal Microscopy
M Quaresma
L Marques

(T3)
RNAi Experiments
B Neumann

(P2)
siRNA design
B Neumann

(T6)
Image Analysis
V Hilsenstein

10.0010.30

Coffee break

Coffee break

Coffee break

Coffee break

Coffee break

10.30‐11.30

(T1) Fluorescence
R Malhó

(L1)
Live cell HT Microscopy
H Botelho
L Marques
R Malhó

(T4)
HTM based RNAi screening
B Neumann

(P2)
siRNA design
B Neumann

(T7)
Statistical analysis
M Antunes
V Hilsenstein

11.3012.00

Coffee break

Coffee break

Coffee break

Coffee break

Coffee break

12.00‐13.00

(T2)
From light to numbers
G Martins

(Seminar 1)
José Rino

(T5)
Assay development
All Faculty

(T5)
Assay development
All Faculty

(Seminar 2)
Margarida Amaral

13.0014.00

Lunch break

Lunch break

Lunch break

Lunch break

Lunch break

14.00‐16.00

(P1)
ImageJ
G Martins
H Botelho

Free afternoon

(L2)
Live cell HT Microscopy
H Botelho
L Marques

(P3)
CellProfiler
V Hilsenstein

(P4)
Data Analysis
H Botelho

16.00‐16.30

Coffee break

Coffee break

Coffee break

Coffee break

16.30‐18.30

(P1)
ImageJ
G Martins
H Botelho

(L2)
Live cell HT Microscopy
H Botelho
L Marques

(P3)
CellProfiler
V Hilsenstein

Final remarks
M Amaral
R Malhó

 

 

Venue

 

HTM 2017 will take place at Faculty of Sciences of the University of Lisbon (FCUL), in Portugal.
Lectures and seminars will be held at C1 and C8 campus buildings, and practical tutorials will be held at the C8 installation of the FCUL Microscopy Facility.

 


Click on the photo for map directions

 

 

Sponsors