Zeiss Axiovert 200

Inverted widefield fluorescence microscope

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PRESENTATION

Axiovert 200

The Zeiss Axiovert 200 is a manual inverted widefield fluorescence microscope. Its sensitive digital camera allows the acquisition of fluorescent signals in light samples and slides. It is suitable for routine examination of cell cultures, manual image acquisition and differential interference contrast (DIC) imaging.


Room: 8.1.79 Computer name: axiovert200 / 10.101.93.112 (why?)


Non-BioISI members: 10 €/h


PosMagNAIMCorr.WD (mm)Glass (mm)Color CodeObs
110x0.25AirA-PLAN4.50.17Ph1, PlasDIC
220x0.80AirPLAN-APO0.550.17-
340x0.6AirLD PLAN-Neofluar2.90-1.5Ph2, PlasDIC

Pos Position

Mag Magnification

NA Numerical Aperture

IM Immersion Medium

Corr. Correction Type

WD Working Distance

Glass Coverglass

Obs Observations


PositionExcitation (nm)Emission (nm)FluorophoresModel
1BP G365 (300-400)BP 420-470DAPI, hoechst, Alexa35049
2BP 450-490BP 515-565GFP, Alexa488, cy2, FITC10
3BP 550-580BP 590-650mCherry, RFP, cy3.5, TRITC31
4BP 625-655BP 665-715Alexa647, Alexa660, cy5, TO-PRO350
5--empty-


Transmitted light

Halogen lamp

Fluorescence

HBO 100

High pressure mercury vapor arc-discharge lamps. Lifetime: 200 hours Includes a dedicated power supply


AxioCam MRm Rev. 3 FireWire

Monochrome CCD camera.

1.4 MP (1388 x 1040 pixels)

Pixel size 6.45 μm × 6.45 μm

Bit depth 8 bit/12 bit

One stage Peltier cooling

Spectral sensitivity range: 350-1000 nm

Integration time: 1 ms – 60 s


Universal supports for for multiwell plates and microscopy slides

Immersion oil (RI 1.5180)


 COMMON USAGE PROCEDURES


Zen 2011

Micro-Manager


 LINKS / USER MANUALS


EQUIPMENT RESPONSIBLE

HugoQ67DIEPD 10922654_10153982169038222_2387869307937053110_o
Hugo Botelho

BioISI

FunGP Group

hmbotelho@fc.ul.pt

Room 8.1.76

Ext: 28176

Luís Marques

BioISI

Microscopy technician

lfmarques@fc.ul.pt

Room 8.1.79

Ext: 28179