Olympus IX50

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PRESENTATION

IX50

Olympus IX50 manual fluorescence microscope with mercury fluorescence illuminator, Nomarski/DIC Prism, cool CCD for fast low-light imaging, motorized filter wheels and micromanipulation / microinjection


Room: 2.1.15 Computer name:


TBA


PosMagNAIMCorr.WD (mm)Glass (mm)Color CodeObs
110x0.40Air / WaterUPlanApo3.10--
220x0.20AirLCPlanFL7.55 - 6.75--
340x0.85AirUPlanApo0.20--
4100x1.25OilPlanC N---


PositionExcitation (nm)Emission (nm)FluorophoresModel
1BP 330-385LP >420DAPI, hoechst
2BP 470-490LP >515GFP, Alexa488, cy2, FITC
3BP 530-550LP >590RFP, YFP, Alexa546, Alexa568, cy3, TRITC


Transmitted light

Halogen lamp

Fluorescence

Olympus U-RFL-T Mercury lamp


PCO Sensicam-QE 12bit


Ludl filter wheels for 6 excitation and 6 emission filters

Narishige micromanipulator


 COMMON USAGE PROCEDURES


[EXPAND SOFTWARE]

Image Pro-Plus with Scope-Pro (control of filter wheels) and Sharpstack (deconvolution module)


LINKS / USER MANUALS


PEOPLE RESPONSIBLE FOR THE EQUIPMENT

Rui Malhó Telmo
Rui Malhó

FCUL / BioISI

Head of Microscopy Facility

r.malho@fc.ul.pt

Room 2.1.44

Ext: 22144

Telmo Nunes

FCUL

Microscopy technician

mevarrimento@fc.ul.pt

Room 2.1.15

Ext: 22181