Leica DMI6000b

Inverted motorized fluorescence microscope for High-Throughput Screening

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PRESENTATIONDMI6000b

The Leica DMI6000 B is a fully automated inverted fluorescence microscope. It features automated contrast and illumination manager, motorized Z focus, automatic brightness and diaphragm adjustment, autofocus, automated multiposition imaging and other automated functions. This system is suitable for high throughput projects and automated imaging in multiwell imaging media. It can accomodate an SP8 confocal module (not installed). This tutorial shows the location and function of some of the major components of the microscope.


Room 8.1.79

Computer name: leicadmi6000b / 10.101.93.14 (why?)


PositionMagnificationNumerical ApertureImmersion MediumCorrectionWD (mm)Glass (mm)Color CodeObservations
15x0.15AirPL S-APO13.7-DIC
210x0.40AirHC PL APO2.20.17
320x0.75Water, glycerol, oilHC PL APO0.68-Correction collar
440x1.10WaterHCX PL APO0.630.14-0.18Correction collar

No phase contrast; DIC ONLY ON 5x


PositionExcitation (nm)Emission (nm)FluorophoresModel
1340-380450-490DAPI, hoechstA
2425-445455-485CFPcustom CFP
3490-510520-550GFP, Alexa488, cy2, FITC, YFPYFP
4515-560>590mCherry, cy3, TRITC, Alexa568N2.1
5630-660>670Alexa 633, Alexa647, cy5custom Cy5
6---DIC ANA + BF


Transmitted light

Halogen lamp

Fluorescence

EL6000 

Mercury metal halide bulb

Lifetime: 2000 hours

Integrated fast shutter


Hamamatsu Orca-Flash4.0

Monochrome CMOS camera 4.0 MP (2048 x 2048 pixels)

Pixel size 6.5 μm × 6.5 μm

Bit depth 16 bit

Peltier cooling


Water immersion micro dispenser

Bartels Mikrotechnik Extended Micropump Controller

Allows continuous water distribution for automated imaging of multiwell plates. Available for all water immersion objectives (20x and 40x).

Universal supports for for multiwell plates and microscopy slides

Immersion oil (RI 1.5180)


COMMON USAGE PROCEDURES


Leica LAS X

Including Leica High Content Screening Software (MatrixScreener)


LINKS / USER MANUALS


BioISI members: Free

Non-BioISI members: 10 €/h


EQUIPMENT RESPONSIBLE

HugoQ67DIEPD 10922654_10153982169038222_2387869307937053110_o
Hugo Botelho

BioISI

FunGP

hmbotelho@fc.ul.pt

Room 8.1.76

Ext: 28176

Luís Marques

BioISI

Microscopy technician

lfmarques@fc.ul.pt

Room 8.1.79

Ext: 28179