ZEISS STEREO LUMAR V.12

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PRESENTATION

01 LUMARv12

Stereo microscope with high contrast and color fidelity. Suitable for embryo observation. Automated control over magnification, focus. Manual control of contrast and brightness. Equipped with metal halide fluorescence illuminator and filter sets.


Room: 2.1.15

Computer name: LumarV12


Zoom range: 10-120x, fully automated; controlled by Zeiss Axiovision v4.8 with multidimensional & extended focus


PositionModelExcitation (nm)Emission (nm)Fluorophores
1Lumar 01BP 365/12LP >397DAPI, Hoescht
2Lumar 38 HEBP 470/40BP 525/50GFP, Alexa488, FITC
3Lumar 15BP 546/12LP >590RFP, Alexa546, Alexa568, TRITC, Cy3
4empty


Transmitted light illuminator

Metal halide fluorescence illuminator


Axiocam  mRm, 1.3MPixels, monochromatic cooled CCD – control and acquisition with Axiovision v4.8

High-speed Casio EX-F1 camera – manual control or with Casio Software

TIS DFK 5MP Camera – acquisition with IC Capture software v2.1


Stage temperature control for mammalian embryo/cells live imaging (by request)


Procedure to turn system on:

  1. PC must be turned on for controller box to turn on
  2. Turn on PC monitor
  3. Turn on power switch for the transmitted light illuminator (front bottom left)
  4. Turn on fluorescence illuminator ONLY IF NECESSARY (eg. to see GFP)
  5. Start software (Axiovision) at the desktop
  6. To preview images click “BF” switch and “live”; to acquire click “snap”.
  7. Save image as ZVI (to maintain calibration) and then convert to TIFF. Open ZVI files with the ImageJ software and the latest version of the LOCI plugin – DO NOT USE THE BUILT IN “ZVI” PLUGIN IN IMAGEJ AS THIS PRODUCES INCORRECTLY CALIBRATED VALUES!!!! Install and use the LOCI plugin instead.

Procedure to turn off:

  1. Turn off LUMAR controller box and transmited light illuminator (and fluorescence)
  2. Save your work and shutdown the PC


Axiovision LE

IC Capture e IC MEasure


Brochure


PEOPLE RESPONSIBLE FOR THE EQUIPMENT

Telmo gabriela_rodrigues
Telmo Nunes

FCUL

Microscopy technician

mevarrimento@fc.ul.pt

Room 2.1.15

Ext: 22181

Gabriela Rodrigues

FCUL / CE3C

Assistant professor

mgrodrigues@fc.ul.pt

Room 2.3.10

Ext: 22310