Zeiss SteREO Lumar V.12

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PRESENTATION

01 LUMARv12

Stereo microscope with high contrast and color fidelity. Suitable for embryo observation. Automated control over magnification, focus. Manual control of contrast and brightness. Equipped with metal halide fluorescence illuminator and filter sets.


Room: 2.1.15

IP: 10.101.134.39 (why?)


Zoom range: 10-120x, fully automated; controlled by Zeiss Axiovision v4.8 with multidimensional & extended focus


PositionModelExcitation (nm)Emission (nm)Fluorophores
1Lumar 01BP 365/12LP >397DAPI, Hoescht
2Lumar 38 HEBP 470/40BP 525/50GFP, Alexa488, FITC
3Lumar 15BP 546/12LP >590RFP, Alexa546, Alexa568, TRITC, Cy3
4empty


Transmitted light illuminator

Metal halide fluorescence illuminator


Zeiss Axiocam 503 color 3MP camera


Stage temperature control for mammalian embryo/cells live imaging (by request)


BASIC PROCEDURE FOR COLOR IMAGES

 

  • Turn on transmitted light power source (1) if you need transmitted light illuminating your sample from below. If you need epi-illumination, turn on the white LED light ring.
  • Turn on Zeiss Lumar V.12 control box (button 2 – check stereoscope powering on on control console blue LCD)
  • Switch between eyepieces and camera port using the right hand side selector. Take care, selector is fragile. Adjust desired magnification and focus, using the control console joystick.
  • Turn on PC and start Axiovision program.

 

 

  • For color images, press button 1 (BF) for empty filterwheel selection.
  • Switch to camera port on the right hand side selector, and press button 2 (Live).
  • Use Linear Histogram (button 3), and click the Overexposure “red pixels” button (button 4). Do not change Camera Properties like Brightness, Contrast, or Gamma, unless you know what you’re doing and wish to do pre-processing.
  • Adjust Camera Exposure (button 5) until the histogram curves barely reach the right of the histogram (white pixels – appear in red in the image). Depending on the kind of illumination or the color of your background, you may need to fiddle with this setting. Always avoid excess white saturation.
  • Click the White Balance Interactive picker (button 6) and click on a blank, white-ish area of your image.
  • Click Snap (button 7) to acquire an image.
  • If you wish to acquire images with proper micrometric calibration for each magnification, you MUST save them in the proprietary Zeiss ZVI format. You can open these in FIJI. Save your images in your image folder.
  • When you’re done with your work, switch everything off in reverse order.

Zeiss Lumar V.12 can also acquire multidimensional images (z-stacks, time lapses, fluorescence channels). If you need these, please ask Telmo or Luís for assistance.


Axiovision 4.81 64bit


Brochure


PEOPLE RESPONSIBLE FOR THE EQUIPMENT

Telmo gabriela_rodrigues
Telmo Nunes

FCUL

Microscopy technician

mevarrimento@fc.ul.pt

Room 2.1.15

Ext: 22181

Gabriela Rodrigues

FCUL / CE3C

Assistant professor

mgrodrigues@fc.ul.pt

Room 2.3.10

Ext: 22310