Leica SP8

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PRESENTATION

The Leica TCS SP8 is a fully motorised high-resolution spectral confocal system,

based on a Leica DMI8 microscope, with High Content Screening capabilities.


Location: 8.1.71

Computer name: leica-sp8st-ws / 10.101.93.35 (why?)


TBA


PosMagNAIMCorr.WDGlassColor CodePixel sizeStep size
110x0.4AirHC Plan Apo2200 um0.17 mm (#1.5)
220x0.75AirHC Plan Apo620 um0.17 mm (#1.5)
363x1.4OilHC Plan Apo140 um0.17 mm (#1.5)
4
5
6

Pos Position

Mag Magnification

NA Numerical Aperture

IM Immersion Medium

Corr. Correction Type

WD Working Distance

Glass Coverglass

Pixel size @ 1024×1024; 1x zoom

Step Confocal section thickness (1 AU)


PositionExcitation (nm)Emission (nm)FluorophoresModel
1450-490LP 515GFP, Alexa488, cy2, FITCI3
2~517- ~562LP 590mCherry, YFP, cy3, Alexa546, Alexa568, TRITCN2.1
3

For eyepieces only!


Transmitted light

White LED

Fluorescence

Mercury metal halide bulb

Lifetime: 2000 hours

Integrated fast shutter

Laser lines

488 nm, 552 nm and 638 nm

ATTENTION: this system does not have a UV laser so DAPI staining cannot be imaged in confocal mode. Use red DNA dyes instead (eg ToPro3, Methyl Green)


3-channel spectral detector + PMTs (400-800nm), 1 transmitted light detector;

(non-normalised spectra; “green” laser is 552)

X Y Z T Lambda acquisition


Immersion oil (RI 1.5180)


Turning system ON:

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Using the system:

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Turning system OFF:

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To open the images you can either use the Leica LAS X software (Windows only) or FIJI.


LINKS / USER MANUALS


PEOPLE RESPONSIBLE FOR THE EQUIPMENT

HugoQ67DIEPD Rui Malhó

Hugo Botelho

BioISI

FunGP

hmbotelho@fc.ul.pt

Room 8.1.76

Ext: 28176

Rui Malhó

FCUL / BioISI

Head of Microscopy Facility

r.malho@fc.ul.pt

Room 2.1.44

Ext: 22144