Hands-on Workshop on High-Throughput Microscopy 2018

 

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Lisboa (Portugal) 9 – 13 July 2018

Limited registrations: 11 participants maximum – REGISTRATIONS CLOSED

Learn about the latest developments on High-Throughput Microscopy, and discuss your project with the experts

 

Contents

Workshop Overview
Participants will learn the basic principles of High-Throughput Microscopy (HTM) applied to RNAi and drug screening. The workshop takes the form of a small HTM project: participants will be introduced to the theory of HTM, involved in discussions on how to design a RNAi/drug screen, perform an actual HTM experiment (including a bioassay and automated image acquisition), perform image analysis and statistical data analysis. Some applications of HTM will be highlighted on research seminars.

Why apply?
Systems biology based approaches are powerful tools to understand complex cellular processes. These approaches rely on the acquisition of extensive datasets in order to characterize a given biological system. Automated high-throughput microscopy technologies allow direct visualization of phenotypes that arise from the treatment of cells/tissues with a large number of siRNAs/compounds/experimental conditions.
This workshop will provide participants with hands-on knowledge on the design, implementation, optimization, image acquisition and analysis of high-throughput microscopy-based screens.

Who should apply?
The workshop is directed at PhD Students, Post-Docs, Microscopy Technicians and Independent Researchers.
Participants are expected to be acquainted with the basics of widefield fluorescence microscopy. No prior experience with high throughput techniques is required.

The course is promoted by BioISI. The instructors are members of FCUL (BioISI, CEAUL) and the Advanced Light Microscopy Facility at EMBL.

Course organizers
Margarida Amaral (BioISI / FCUL)
Rui Malhó (BioISI / FCUL)
Hugo Botelho (BioISI / FCUL)

 

 

Additional Information

Lab work

Perform a screening experiment, get image data, analyze results and discuss the outcome

Hands-on siRNA screening experiment:

  • Cell seeding
  • siRNA transfection
  • Immunofluorescence
  • Automated image acquisition
  • High content analysis
  • Data inspection, visualization and normalization
  • Discussion

Paper presentations

Discussion of high-throughput screening papers published

The authors of some of the papers will be present

Participant presentations

Short presentation (5 min) of each participant’s work

Self-introduction and motivation for discussion/interactions during the course

Assay development session

Interactive discussion on the design of new microscopy-based assays

All faculty will be available

Participants wanting to get advice will be asked to request a time slot

Informal discussions

Instructors will be available for informal 1:1 discussions during the course

Instrument demos

Bring own samples

Instruments will be available during the whole course

Available microscopes:

Leica DMI6000B (automated widefield fluorescence microscope)

Leica SP8 (automated confocal microscope)

Zeiss CellDiscoverer (widefield high content microscope)

 

Faculty and Schedule

Margarida Amaral
FCUL, Lisboa
Rui
Malhó

FCUL, Lisboa
Hugo Botelho
FCUL, Lisboa
Rainer Pepperkok 
EMBL, Heidelberg
Beate Neumann
EMBL, Heidelberg
Christian Tischer
EMBL, Heidelberg
Luís Marques
FCUL, Lisboa
       

 

Venue

The workshop will take place at Faculty of Sciences of the University of Lisbon (FCUL), in Portugal.
Lectures and seminars will be held at C1 and C8 campus buildings, and practical tutorials will be held at the C8 installation of the FCUL Microscopy Facility.

 


Click on the photo for map directions

 

 

Registration

 

Registrations closed

 

Sponsors

 

 
 

 

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